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goat anti human prox  (R&D Systems)


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    Structured Review

    R&D Systems goat anti human prox
    Goat Anti Human Prox, supplied by R&D Systems, used in various techniques. Bioz Stars score: 95/100, based on 323 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/goat+anti+human+prox+1/Human+Prox1+Antibody/bio_rxiv__2025__01__15__631989-313-23-27
    Average 95 stars, based on 323 article reviews
    goat anti human prox - by Bioz Stars, 2026-10
    95/100 stars

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    Related Articles

    Incubation:

    Article Title: IL-7 enhances the differentiation of adipose-derived stem cells toward lymphatic endothelial cells through AKT signaling.
    Article Snippet: Our study was designed to investigate the effects of IL-7 during the differentiation process of adipose-derived stem cells (ADSCs) toward lymphatic endothelial cells (LECs).. IL-7 was added to the traditional induced medium, which was called the IL-7 (+) group, while the group that used traditional induced medium was called the IL-7 (-) group.. After 7 days of induction of ADSCs, a comprehensive analysis was conducted between these two groups.

    Article Title: Generation of pure lymphatic endothelial cells from human pluripotent stem cells and their therapeutic effects on wound repair
    Article Snippet: For four-marker staining, cells were incubated with Alexa488-conjugated rat anti-human PODOPLANIN (Biolegend), PE-conjugated mouse anti-human VEGFR3 (R&D system), and biotinylated rabbit anti-human LYVE-1 (ReliaTech GmbH) followed by streptavidin PE-Cy5 (BD Pharminogen). .. After washing, the cells were fixed/permeabilized (IC Fixation/Permeabilization buffer, eBioscience) and incubated with goat anti-human PROX-1 (R&D Systems), followed by donkey APC-Cy7-conjugated anti-goat IgG (Santa cruz). .. The resulting cells were analyzed on a C6 Flow Cytometer (Accuri Cytometers Inc) and data were evaluated with CFlow software.

    Immunohistochemistry:

    Article Title: CXCL5 Facilitates Melanoma Cell-Neutrophil Interaction and Lymph Node Metastasis.
    Article Snippet: .. Immunohistochemistry, immunofluorescence and flow cytometry Primary antibodies: Mouse anti-human VIMENTIN Clone V9 (#M0725; Dako, Glostrup, Denmark), rabbit anti-mouse LYVE-1 (#DP3513; Acris, Herford, Germany), rabbit anti-mouse CD31 (#Rb10333; Neomarkers, Fremont, CA), mouse anti-human CXCL5 Clone 33160 (#MAB254; R&D Minneapolis, MN), rat anti-mouse LY6G Clone 1A8 (#551459; BD Pharmingen, San Diego, CA), mouse anti-human CD66b Clone G10F5 (#555723; Pharmingen, San Diego, CA), mouse anti-human CXCR2 Clone ab24963 (#ab24963; Abcam,Cambridge, UK), goat anti-human Prox-1 (#AF2727; R&D Minneapolis, MN). .. Paraffin sections were deparaffinized followed by antigen retrieval by boiling in citrate buffer (DAKO, Vienna, Austria).

    Immunofluorescence:

    Article Title: CXCL5 Facilitates Melanoma Cell-Neutrophil Interaction and Lymph Node Metastasis.
    Article Snippet: .. Immunohistochemistry, immunofluorescence and flow cytometry Primary antibodies: Mouse anti-human VIMENTIN Clone V9 (#M0725; Dako, Glostrup, Denmark), rabbit anti-mouse LYVE-1 (#DP3513; Acris, Herford, Germany), rabbit anti-mouse CD31 (#Rb10333; Neomarkers, Fremont, CA), mouse anti-human CXCL5 Clone 33160 (#MAB254; R&D Minneapolis, MN), rat anti-mouse LY6G Clone 1A8 (#551459; BD Pharmingen, San Diego, CA), mouse anti-human CD66b Clone G10F5 (#555723; Pharmingen, San Diego, CA), mouse anti-human CXCR2 Clone ab24963 (#ab24963; Abcam,Cambridge, UK), goat anti-human Prox-1 (#AF2727; R&D Minneapolis, MN). .. Paraffin sections were deparaffinized followed by antigen retrieval by boiling in citrate buffer (DAKO, Vienna, Austria).

    Flow Cytometry:

    Article Title: CXCL5 Facilitates Melanoma Cell-Neutrophil Interaction and Lymph Node Metastasis.
    Article Snippet: .. Immunohistochemistry, immunofluorescence and flow cytometry Primary antibodies: Mouse anti-human VIMENTIN Clone V9 (#M0725; Dako, Glostrup, Denmark), rabbit anti-mouse LYVE-1 (#DP3513; Acris, Herford, Germany), rabbit anti-mouse CD31 (#Rb10333; Neomarkers, Fremont, CA), mouse anti-human CXCL5 Clone 33160 (#MAB254; R&D Minneapolis, MN), rat anti-mouse LY6G Clone 1A8 (#551459; BD Pharmingen, San Diego, CA), mouse anti-human CD66b Clone G10F5 (#555723; Pharmingen, San Diego, CA), mouse anti-human CXCR2 Clone ab24963 (#ab24963; Abcam,Cambridge, UK), goat anti-human Prox-1 (#AF2727; R&D Minneapolis, MN). .. Paraffin sections were deparaffinized followed by antigen retrieval by boiling in citrate buffer (DAKO, Vienna, Austria).

    other:

    Article Title: Myeloid Cells Contribute to Tumor Lymphangiogenesis
    Article Snippet: The following primary antibodies were used at the dilutions specified in brackets: rat anti-mouse LYVE-1 (1∶200) (Clone ALY-7, MBL, Japan), rabbit anti-mouse LYVE-1 (1∶200) (Reliatech, Germany), rabbit anti-mouse Prox-1 (1∶100) (K. Alitalo, University of Helsinki), goat anti-human Prox-1 (1∶100) (R&D Systems), rabbit anti-Podoplanin (1∶100) (D. Kerjaschki, Medical University Vienna), hamster anti-mouse Podoplanin hybridoma supernatant (1∶20) (Clone 8.1.1), rat anti-mouse VE-Cadherin hybridoma supernatant (1∶50) (Clone B14, E. Dejana, University of Milano), rat anti-mouse F4/80 (1∶200) (Clone CI:A3-1, Serotec), rat anti-mouse CD11b (1∶100) (Clone M1/70.15, Serotec) and rat anti-mouse CD31 (1∶50) (Clone MEC 13.3, Pharmingen).



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    Lymphvasculogenesis constitute a cellular process for ocular lymphatic vessel development. ( A ) Visualization of the presence of a LYVE-1+ <t>Prox-1+</t> cell cluster ( box ) at the P0 ocular surface after whole mount immunofluorescent staining of the eye anterior segment and flat-mounting. The yellow asterisks mark the location of two lymphatic sprouts. The green arrows point to some isolated LYVE-1–positive cells that correspond to macrophages. ( B , C ) Imaging of the boxed LYVE-1+ Prox-1+ lymphatic cell cluster at a higher magnification. ( D ) LSFM imaging of some LYVE-1-positive lymphatic vessel fragments ( yellow arrows ), which have formed at the corneal and corneolimbal surfaces of a P1 neonate. Co, cornea. ( E ) LYVE-1 immunofluorescence staining image obtained at P1, allowing the view of both lymphangiogenic sprouting (sprout extremities are pointed by white arrows ), and a lymphatic vessel fragment, which could have been formed by lymphvasculogenesis ( yellow arrow ).
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    Lymphvasculogenesis constitute a cellular process for ocular lymphatic vessel development. ( A ) Visualization of the presence of a LYVE-1+ <t>Prox-1+</t> cell cluster ( box ) at the P0 ocular surface after whole mount immunofluorescent staining of the eye anterior segment and flat-mounting. The yellow asterisks mark the location of two lymphatic sprouts. The green arrows point to some isolated LYVE-1–positive cells that correspond to macrophages. ( B , C ) Imaging of the boxed LYVE-1+ Prox-1+ lymphatic cell cluster at a higher magnification. ( D ) LSFM imaging of some LYVE-1-positive lymphatic vessel fragments ( yellow arrows ), which have formed at the corneal and corneolimbal surfaces of a P1 neonate. Co, cornea. ( E ) LYVE-1 immunofluorescence staining image obtained at P1, allowing the view of both lymphangiogenic sprouting (sprout extremities are pointed by white arrows ), and a lymphatic vessel fragment, which could have been formed by lymphvasculogenesis ( yellow arrow ).
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    Lymphvasculogenesis constitute a cellular process for ocular lymphatic vessel development. ( A ) Visualization of the presence of a LYVE-1+ <t>Prox-1+</t> cell cluster ( box ) at the P0 ocular surface after whole mount immunofluorescent staining of the eye anterior segment and flat-mounting. The yellow asterisks mark the location of two lymphatic sprouts. The green arrows point to some isolated LYVE-1–positive cells that correspond to macrophages. ( B , C ) Imaging of the boxed LYVE-1+ Prox-1+ lymphatic cell cluster at a higher magnification. ( D ) LSFM imaging of some LYVE-1-positive lymphatic vessel fragments ( yellow arrows ), which have formed at the corneal and corneolimbal surfaces of a P1 neonate. Co, cornea. ( E ) LYVE-1 immunofluorescence staining image obtained at P1, allowing the view of both lymphangiogenic sprouting (sprout extremities are pointed by white arrows ), and a lymphatic vessel fragment, which could have been formed by lymphvasculogenesis ( yellow arrow ).
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    R&D Systems goat anti prox 1
    Lymphvasculogenesis constitute a cellular process for ocular lymphatic vessel development. ( A ) Visualization of the presence of a LYVE-1+ <t>Prox-1+</t> cell cluster ( box ) at the P0 ocular surface after whole mount immunofluorescent staining of the eye anterior segment and flat-mounting. The yellow asterisks mark the location of two lymphatic sprouts. The green arrows point to some isolated LYVE-1–positive cells that correspond to macrophages. ( B , C ) Imaging of the boxed LYVE-1+ Prox-1+ lymphatic cell cluster at a higher magnification. ( D ) LSFM imaging of some LYVE-1-positive lymphatic vessel fragments ( yellow arrows ), which have formed at the corneal and corneolimbal surfaces of a P1 neonate. Co, cornea. ( E ) LYVE-1 immunofluorescence staining image obtained at P1, allowing the view of both lymphangiogenic sprouting (sprout extremities are pointed by white arrows ), and a lymphatic vessel fragment, which could have been formed by lymphvasculogenesis ( yellow arrow ).
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    Image Search Results


    Lymphvasculogenesis constitute a cellular process for ocular lymphatic vessel development. ( A ) Visualization of the presence of a LYVE-1+ Prox-1+ cell cluster ( box ) at the P0 ocular surface after whole mount immunofluorescent staining of the eye anterior segment and flat-mounting. The yellow asterisks mark the location of two lymphatic sprouts. The green arrows point to some isolated LYVE-1–positive cells that correspond to macrophages. ( B , C ) Imaging of the boxed LYVE-1+ Prox-1+ lymphatic cell cluster at a higher magnification. ( D ) LSFM imaging of some LYVE-1-positive lymphatic vessel fragments ( yellow arrows ), which have formed at the corneal and corneolimbal surfaces of a P1 neonate. Co, cornea. ( E ) LYVE-1 immunofluorescence staining image obtained at P1, allowing the view of both lymphangiogenic sprouting (sprout extremities are pointed by white arrows ), and a lymphatic vessel fragment, which could have been formed by lymphvasculogenesis ( yellow arrow ).

    Journal: Investigative Ophthalmology & Visual Science

    Article Title: Ontogenesis of the Mouse Ocular Surface Lymphatic Vascular Network

    doi: 10.1167/iovs.64.15.7

    Figure Lengend Snippet: Lymphvasculogenesis constitute a cellular process for ocular lymphatic vessel development. ( A ) Visualization of the presence of a LYVE-1+ Prox-1+ cell cluster ( box ) at the P0 ocular surface after whole mount immunofluorescent staining of the eye anterior segment and flat-mounting. The yellow asterisks mark the location of two lymphatic sprouts. The green arrows point to some isolated LYVE-1–positive cells that correspond to macrophages. ( B , C ) Imaging of the boxed LYVE-1+ Prox-1+ lymphatic cell cluster at a higher magnification. ( D ) LSFM imaging of some LYVE-1-positive lymphatic vessel fragments ( yellow arrows ), which have formed at the corneal and corneolimbal surfaces of a P1 neonate. Co, cornea. ( E ) LYVE-1 immunofluorescence staining image obtained at P1, allowing the view of both lymphangiogenic sprouting (sprout extremities are pointed by white arrows ), and a lymphatic vessel fragment, which could have been formed by lymphvasculogenesis ( yellow arrow ).

    Article Snippet: Goat anti-mouse LYVE-1 (AF2125), rat anti-mouse LYVE-1 (MAB2125), goat anti-mouse Prox-1 (AF2727), and goat anti-mouse CD80 (AF740) were obtained from Biotechne (Minneapolis, MN, USA).

    Techniques: Staining, Isolation, Imaging, Immunofluorescence

    Evidence for the existence of mixed Prox-1– and CD206-positive cells in lymphatic neovessels. Whole mount double immunofluorescence staining with LYVE-1 and CD206 antibodies ( A–C ) or with Prox-1 and CD206 antibodies ( D–F ), of the developing ocular surface lymphatic vasculature at P1. The yellow asterisk marks a CD206-positive macrophage displaying a low LYVE-1 expression when compared to the LYVE-1 expression level of cells constituting the lymphatic neovessel. The white arrows point to the CD206 immunoreactivity in some LYVE-1– ( upper panels ) or Prox-1–positive cells ( lower panels ) of the lymphatic neovessel.

    Journal: Investigative Ophthalmology & Visual Science

    Article Title: Ontogenesis of the Mouse Ocular Surface Lymphatic Vascular Network

    doi: 10.1167/iovs.64.15.7

    Figure Lengend Snippet: Evidence for the existence of mixed Prox-1– and CD206-positive cells in lymphatic neovessels. Whole mount double immunofluorescence staining with LYVE-1 and CD206 antibodies ( A–C ) or with Prox-1 and CD206 antibodies ( D–F ), of the developing ocular surface lymphatic vasculature at P1. The yellow asterisk marks a CD206-positive macrophage displaying a low LYVE-1 expression when compared to the LYVE-1 expression level of cells constituting the lymphatic neovessel. The white arrows point to the CD206 immunoreactivity in some LYVE-1– ( upper panels ) or Prox-1–positive cells ( lower panels ) of the lymphatic neovessel.

    Article Snippet: Goat anti-mouse LYVE-1 (AF2125), rat anti-mouse LYVE-1 (MAB2125), goat anti-mouse Prox-1 (AF2727), and goat anti-mouse CD80 (AF740) were obtained from Biotechne (Minneapolis, MN, USA).

    Techniques: Double Immunofluorescence Staining, Expressing